Characterization and functional study of a chimera galectin from yellow drum Nibea albiflora.
Int J Biol Macromol
; 187: 361-372, 2021 Sep 30.
Article
in En
| MEDLINE
| ID: mdl-34314796
ABSTRACT
Galectins are protein that participates in a variety of immune responses in the process of pathogenic infections. In the present study, a chimera galectin gene was screened from the transcriptome database of Nibea albiflora, which was named as YdGal-3. The results of qRT-PCR showed that the mRNA transcripts of YdGal-3 were ubiquitously distributed in all the detected tissues. After infection with Vibrio harveyi, the expression of YdGal-3 in liver, spleen, and head kidney increased significantly. Immunohistochemistry showed that YdGal-3 protein was widely expressed in the head kidney. The purified YdGal-3 protein by prokaryotic expression agglutinated red blood cells. Sugar inhibition assay showed that the agglutinating activity of YdGal-3 protein was inhibited by different sugars including lactose, D-galactose, and lipopolysaccharide. In addition, we mutated YdGal-3 His 294 into proline (P), alanine (A), glycine (G), and aspartic acid (D), it was further proved that the residue plays a key role in agglutination. YdGal-3 agglutinated some gram-negative bacteria including Pseudomonas plecoglossicida, Vibrio parahemolyticus, V. harveyi, and Aeromonas hydrophila, and exhibited antibacterial activity. These results suggested that YdGal-3 protein played an important role in the innate immunity of N. albiflora.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vibrio
/
Vibrio Infections
/
Fish Proteins
/
Galectin 3
/
Fish Diseases
/
Fishes
/
Immunity, Innate
Limits:
Animals
Language:
En
Journal:
Int J Biol Macromol
Year:
2021
Document type:
Article
Affiliation country:
China