Serodiagnosis and Bacterial Genome of Helicobacter pylori Infection.
Toxins (Basel)
; 13(7)2021 07 05.
Article
in En
| MEDLINE
| ID: mdl-34357939
ABSTRACT
The infection caused by Helicobacter pylori is associated with several diseases, including gastric cancer. Several methods for the diagnosis of H. pylori infection exist, including endoscopy, the urea breath test, and the fecal antigen test, which is the serum antibody titer test that is often used since it is a simple and highly sensitive test. In this context, this study aims to find the association between different antibody reactivities and the organization of bacterial genomes. Next-generation sequences were performed to determine the genome sequences of four strains of antigens with different reactivity. The search was performed on the common genes, with the homology analysis conducted using a genome ring and dot plot analysis. The two antigens of the highly reactive strains showed a high gene homology, and Western blots for CagA and VacA also showed high expression levels of proteins. In the poorly responsive antigen strains, it was found that the inversion occurred around the vacA gene in the genome. The structure of bacterial genomes might contribute to the poor reactivity exhibited by the antibodies of patients. In the future, an accurate serodiagnosis could be performed by using a strain with few gene mutations of the antigen used for the antibody titer test of H. pylori.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Serologic Tests
/
Helicobacter pylori
/
Helicobacter Infections
/
Genome, Bacterial
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Toxins (Basel)
Year:
2021
Document type:
Article
Affiliation country:
Japan