Targeted truncated TGF-ß receptor type II delivery to fibrotic liver by PDGFß receptor-binding peptide modification for improving the anti-fibrotic activity against hepatic fibrosis in vitro and in vivo.
Int J Biol Macromol
; 188: 941-949, 2021 Oct 01.
Article
in En
| MEDLINE
| ID: mdl-34389395
ABSTRACT
Truncated transforming growth factor-ß receptor type II (tTßRII) is a promising anti-fibrotic candidate because it attenuates excessive transforming growth factor-ß1 (TGF-ß1) and then blocks TGF-ß1 activity in hepatic fibrosis. However, its use has been greatly limited due to the fact that it is expensive to chemically synthesize and it does not specifically target to the lesion site. In this study, we describe that platelet-derived growth factor ß receptor (PDGFßR)-binding peptide BiPPB modified tTßRII (BiPPB-tTßRII) was prepared from the cleavage of SUMO-BiPPB-tTßRII by digestion with SUMO-specific protease. Moreover, compared to the unmodified tTßRII, the target protein BiPPB-tTßRII not only highly specific targeted activated hepatic stellate cells (HSCs) and fibrotic liver tissue, but also significantly inhibited the protein levels of fibrosis-related genes in TGF-ß1-induced HSC-T6 cells and CCl4-induced liver fibrosis in mice. Furthermore, BiPPB-tTßRII markedly ameliorated liver morphology, fibrotic responses and the damage of liver function in fibrosis animal. More importantly, BiPPB-tTßRII showed a much lesser extent in binding to quiescent HSCs and non-fibrotic liver tissue. Taken together, our results suggested that the target protein BiPPB-tTßRII, with its high specific fibrotic liver-targeting potential and its improved anti-fibrotic activity in liver fibrosis, may be a potential therapeutic agent for liver fibrosis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Receptor, Platelet-Derived Growth Factor beta
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Receptor, Transforming Growth Factor-beta Type II
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Liver Cirrhosis
Limits:
Animals
Language:
En
Journal:
Int J Biol Macromol
Year:
2021
Document type:
Article