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Transition-Metal Dichalcogenide Artificial Antibodies with Multivalent Polymeric Recognition Phases for Rapid Detection and Inactivation of Pathogens.
Lee, Sin; Kang, Tae Woog; Hwang, In-Jun; Kim, Hye-In; Jeon, Su-Ji; Yim, DaBin; Choi, Chanhee; Son, Wooic; Kim, Hyunsung; Yang, Chul-Su; Lee, Hwankyu; Kim, Jong-Ho.
Affiliation
  • Lee S; Department of Materials Science and Chemical Engineering, Hanyang University, Ansan 15588, Republic of Korea.
  • Kang TW; Department of Materials Science and Chemical Engineering, Hanyang University, Ansan 15588, Republic of Korea.
  • Hwang IJ; Department of Materials Science and Chemical Engineering, Hanyang University, Ansan 15588, Republic of Korea.
  • Kim HI; Department of Materials Science and Chemical Engineering, Hanyang University, Ansan 15588, Republic of Korea.
  • Jeon SJ; Department of Materials Science and Chemical Engineering, Hanyang University, Ansan 15588, Republic of Korea.
  • Yim D; Department of Materials Science and Chemical Engineering, Hanyang University, Ansan 15588, Republic of Korea.
  • Choi C; Department of Materials Science and Chemical Engineering, Hanyang University, Ansan 15588, Republic of Korea.
  • Son W; Department of Molecular and Life Science and Center for Bionano Intelligence Education and Research, Hanyang University, Ansan 15588, Republic of Korea.
  • Kim H; Department of Pathology, Hanyang University College of Medicine, Seoul 04763, Republic of Korea.
  • Yang CS; Department of Molecular and Life Science and Center for Bionano Intelligence Education and Research, Hanyang University, Ansan 15588, Republic of Korea.
  • Lee H; Department of Chemical Engineering, Dankook University, Yongin 16890, Republic of Korea.
  • Kim JH; Department of Materials Science and Chemical Engineering, Hanyang University, Ansan 15588, Republic of Korea.
J Am Chem Soc ; 143(36): 14635-14645, 2021 09 15.
Article in En | MEDLINE | ID: mdl-34410692
ABSTRACT
Antibodies are recognition molecules that can bind to diverse targets ranging from pathogens to small analytes with high binding affinity and specificity, making them widely employed for sensing and therapy. However, antibodies have limitations of low stability, long production time, short shelf life, and high cost. Here, we report a facile approach for the design of luminescent artificial antibodies with nonbiological polymeric recognition phases for the sensitive detection, rapid identification, and effective inactivation of pathogenic bacteria. Transition-metal dichalcogenide (TMD) nanosheets with a neutral dextran phase at the interfaces selectively recognized S. aureus, whereas the nanosheets bearing a carboxymethylated dextran phase selectively recognized E. coli O157H7 with high binding affinity. The bacterial binding sites recognized by the artificial antibodies were thoroughly identified by experiments and molecular dynamics simulations, revealing the significance of their multivalent interactions with the bacterial membrane components for selective recognition. The luminescent WS2 artificial antibodies could rapidly detect the bacteria at a single copy from human serum without any purification and amplification. Moreover, the MoSe2 artificial antibodies selectively killed the pathogenic bacteria in the wounds of infected mice under light irradiation, leading to effective wound healing. This work demonstrates the potential of TMD artificial antibodies as an alternative to antibodies for sensing and therapy.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Luminescent Agents / Nanostructures / Escherichia coli Infections / Anti-Bacterial Agents Type of study: Diagnostic_studies Language: En Journal: J Am Chem Soc Year: 2021 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Luminescent Agents / Nanostructures / Escherichia coli Infections / Anti-Bacterial Agents Type of study: Diagnostic_studies Language: En Journal: J Am Chem Soc Year: 2021 Document type: Article