A paper-based colorimetric molecular test for SARS-CoV-2 in saliva.
Biosens Bioelectron X
; 9: 100076, 2021 Dec.
Article
in En
| MEDLINE
| ID: mdl-34423284
ABSTRACT
Herein, we describe the development of a paper-based device to detect nucleic acids of pathogens of interest in complex samples using loop-mediated isothermal amplification (LAMP) by producing a colorimetric response visible to the human eye. To demonstrate the utility of this device in emerging public health emergencies, we developed and optimized our device to detect SARS-CoV-2 in human saliva without preprocessing. The resulting device was capable of detecting the virus within 60 min and had an analytical sensitivity of 97% and a specificity of 100% with a limit of detection of 200 genomic copies/µL of patient saliva using image analysis. The device consists of a configurable number of reaction zones constructed of Grade 222 chromatography paper separated by 20 mil polystyrene spacers attached to a Melinex® backing via an ARclean® double-sided adhesive. The resulting device is easily configurable to detect multiple targets and has the potential to detect a variety of pathogens simply by changing the LAMP primer sets.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Biosens Bioelectron X
Year:
2021
Document type:
Article
Affiliation country:
United States