Two Nicotiana occidentalis accessions enable gene identification for Type II hybrid lethality by the cross to N. sylvestris.
Sci Rep
; 11(1): 17093, 2021 08 24.
Article
in En
| MEDLINE
| ID: mdl-34429461
ABSTRACT
Hybrid lethality, meaning the death of F1 hybrid seedlings, has been observed in many plant species, including Nicotiana. Previously, we have revealed that hybrids of the selected Nicotiana occidentalis accession and N. tabacum, an allotetraploid with S and T genomes, exhibited lethality characterized by the fading of shoot color. The lethality was suggested to be controlled by alleles of loci on the S and T genomes derived from N. sylvestris and N. tomentosiformis, respectively. Here, we extended the analysis of hybrid lethality using other two accessions of N. occidentalis identified from the five tested accessions. The two accessions were crossed with N. tabacum and its two progenitors, N. sylvestris and N. tomentosiformis. After crosses with N. tabacum, the two N. occidentalis accessions yielded inviable hybrid seedlings whose lethality was characterized by the fading of shoot color, but only the T genome of N. tabacum was responsible for hybrid lethality. Genetic analysis indicated that first-mentioned N. occidentalis accession carries a single gene causing hybrid lethality by allelic interaction with the S genome.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nicotiana
/
Genes, Lethal
/
Hybridization, Genetic
Type of study:
Diagnostic_studies
Language:
En
Journal:
Sci Rep
Year:
2021
Document type:
Article
Affiliation country:
Japan