Generation of ovarian follicles from mouse pluripotent stem cells.
Science
; 373(6552)2021 07 16.
Article
in En
| MEDLINE
| ID: mdl-34437124
Oocytes mature in a specialized fluid-filled sac, the ovarian follicle, which provides signals needed for meiosis and germ cell growth. Methods have been developed to generate functional oocytes from pluripotent stem cell-derived primordial germ cell-like cells (PGCLCs) when placed in culture with embryonic ovarian somatic cells. In this study, we developed culture conditions to recreate the stepwise differentiation process from pluripotent cells to fetal ovarian somatic cell-like cells (FOSLCs). When FOSLCs were aggregated with PGCLCs derived from mouse embryonic stem cells, the PGCLCs entered meiosis to generate functional oocytes capable of fertilization and development to live offspring. Generating functional mouse oocytes in a reconstituted ovarian environment provides a method for in vitro oocyte production and follicle generation for a better understanding of mammalian reproduction.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oocytes
/
Oogenesis
/
Mouse Embryonic Stem Cells
/
Ovarian Follicle
Limits:
Animals
Language:
En
Journal:
Science
Year:
2021
Document type:
Article
Affiliation country:
Japan
Country of publication:
United States