Exosome-Mediated Activation of Neuronal Cells Triggered by γ-Aminobutyric Acid (GABA).
Nutrients
; 13(8)2021 Jul 25.
Article
in En
| MEDLINE
| ID: mdl-34444704
ABSTRACT
γ-Aminobutyric acid (GABA) is a potent bioactive amino acid, and several studies have shown that oral administration of GABA induces relaxation, improves sleep, and reduces psychological stress and fatigue. In a recent study, we reported that exosomes derived from GABA-treated intestinal cells serve as signal transducers that mediate brain-gut interactions. Therefore, the purpose of this study was to verify the functionality of GABA-derived exosomes and to examine the possibility of improving memory function following GABA administration. The results showed that exosomes derived from GABA-treated intestinal cells (Caco-2) activated neuronal cells (SH-SY5Y) by regulating genes related to neuronal cell functions. Furthermore, we found that exosomes derived from the serum of GABA-treated mice also activated SH-SY5Y cells, indicating that exosomes, which are capable of activating neuronal cells, circulate in the blood of mice orally administered GABA. Finally, we performed a microarray analysis of mRNA isolated from the hippocampus of mice that were orally administered GABA. The results revealed changes in the expression of genes related to brain function. Gene Set Enrichment Analysis (GSEA) showed that oral administration of GABA affected the expression of genes related to memory function in the hippocampus.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Exosomes
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Gamma-Aminobutyric Acid
/
Memory
/
Neurons
Limits:
Animals
/
Humans
Language:
En
Journal:
Nutrients
Year:
2021
Document type:
Article
Affiliation country:
Japan
Publication country:
CH
/
SUIZA
/
SUÍÇA
/
SWITZERLAND