Down-regulated placental miR-21 contributes to preeclampsia through targeting RASA1.
Hypertens Pregnancy
; 40(3): 236-245, 2021 Aug.
Article
in En
| MEDLINE
| ID: mdl-34464226
ABSTRACT
Human placenta was obtained from early onset preeclampsia, late onset preeclampsia, and their gestational age-matched normal pregnancy. Using RT-qPCR, western blot, and immunohistochemistry, it was demonstrated that miR-21 expressions were significantly decreased in preeclampsia while RASA1 were increased. Suppression of miR-21 in placental HTR-8/SVneo cells, remarkably upregulated RASA1, decreased proliferation, inhibited invasion, and promoted apoptosis of trophoblast cells, while overexpression of miR-21 alleviated these effects. Dual-luciferase reporter assays revealed RASA1 to be a direct target of miR-21 in trophoblast cells. miR-21 may serve key roles in the development of preeclampsia by targeting RASA1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pre-Eclampsia
/
P120 GTPase Activating Protein
/
MicroRNAs
Limits:
Adult
/
Female
/
Humans
/
Pregnancy
Language:
En
Journal:
Hypertens Pregnancy
Journal subject:
ANGIOLOGIA
/
OBSTETRICIA
Year:
2021
Document type:
Article
Affiliation country:
China