A Cell Culture Model of BK Polyomavirus Persistence, Genome Recombination, and Reactivation.
mBio
; 12(5): e0235621, 2021 10 26.
Article
in En
| MEDLINE
| ID: mdl-34473564
ABSTRACT
BK polyomavirus (BKPyV) is a small nonenveloped DNA virus that establishes a ubiquitous, asymptomatic, and lifelong persistent infection in at least 80% of the world's population. In some immunosuppressed transplant recipients, BKPyV reactivation causes polyomavirus-associated nephropathy and hemorrhagic cystitis. We report a novel in vitro model of BKPyV persistence and reactivation using a BKPyV natural host cell line. In this system, viral genome loads remain constant for various times after establishment of persistent infection, during which BKPyV undergoes extensive random genome recombination. Certain recombination events result in viral DNA amplification and protein expression, resulting in production of viruses with enhanced replication ability. IMPORTANCE BK polyomavirus (BKPyV) generally establishes a persistent subclinical infection in healthy individuals but can cause severe disease in transplant recipients. While an in vitro model to study acute replication exists, no practical model with which to study BKPyV persistence is currently available. We established a BKPyV persistence model in cell culture. Our model reveals that the virus can persist for various periods of time before random recombination of the viral genome leads to enhanced replication.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Virus Activation
/
Genome, Viral
/
BK Virus
/
Cell Culture Techniques
/
Polyomavirus Infections
/
Persistent Infection
Type of study:
Evaluation_studies
Limits:
Humans
Language:
En
Journal:
MBio
Year:
2021
Document type:
Article
Affiliation country:
United States