Cytogenetics from the Dock: A New Postmortem Protocol Makes It Possible to Obtain Cytogenetic Preparations from Marine Fish.
Zebrafish
; 18(5): 326-329, 2021 10.
Article
in En
| MEDLINE
| ID: mdl-34491117
ABSTRACT
Cytogenetic studies in marine fish are scarce, and elemental cytogenetic information is available for not >2% of the species. Traditional cytogenetic methods require living individuals for their application, making the analysis of marine ichthyofauna very difficult. In this study, we present a detailed new protocol to obtain cytogenetic preparations from marine fish, through access to specimens in postmortem condition. The application of this protocol made it possible to access elemental cytogenetic information (diploid number) in six native species of the South Pacific Ocean, representative of five orders. In this way, we provide a new low-cost methodological tool for focused or large-scale cytogenetic analysis, both in economically important, native, or threatened species.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Zebrafish
Limits:
Animals
Language:
En
Journal:
Zebrafish
Journal subject:
BIOLOGIA
Year:
2021
Document type:
Article
Affiliation country:
Chile
Publication country:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA