Your browser doesn't support javascript.
loading
Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.
Yu, Fangjun; Wang, Zhigang; Zhang, Tianpeng; Chen, Xun; Xu, Haiman; Wang, Fei; Guo, Lianxia; Chen, Min; Liu, Kaisheng; Wu, Baojian.
Affiliation
  • Yu F; Institute of Molecular Rhythm and Metabolism, Guangzhou University of Chinese Medicine, Guangzhou, China.
  • Wang Z; College of Pharmacy, Jinan University, Guangzhou, China.
  • Zhang T; Department of Intensive Care Unit, First Affiliated Hospital of Jinan University, Guangzhou, China.
  • Chen X; Institute of Molecular Rhythm and Metabolism, Guangzhou University of Chinese Medicine, Guangzhou, China.
  • Xu H; College of Pharmacy, Jinan University, Guangzhou, China.
  • Wang F; College of Pharmacy, Jinan University, Guangzhou, China.
  • Guo L; College of Pharmacy, Jinan University, Guangzhou, China.
  • Chen M; College of Pharmacy, Jinan University, Guangzhou, China.
  • Liu K; College of Pharmacy, Jinan University, Guangzhou, China.
  • Wu B; Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, Shenzhen, China. kaisheng_liu@163.com.
Nat Commun ; 12(1): 5323, 2021 09 07.
Article in En | MEDLINE | ID: mdl-34493722
ABSTRACT
The role of intestine clock in energy homeostasis remains elusive. Here we show that mice with Bmal1 specifically deleted in the intestine (Bmal1iKO mice) have a normal phenotype on a chow diet. However, on a high-fat diet (HFD), Bmal1iKO mice are protected against development of obesity and related abnormalities such as hyperlipidemia and fatty livers. These metabolic phenotypes are attributed to impaired lipid resynthesis in the intestine and reduced fat secretion. Consistently, wild-type mice fed a HFD during nighttime (with a lower BMAL1 expression) show alleviated obesity compared to mice fed ad libitum. Mechanistic studies uncover that BMAL1 transactivates the Dgat2 gene (encoding the triacylglycerol synthesis enzyme DGAT2) via direct binding to an E-box in the promoter, thereby promoting dietary fat absorption. Supporting these findings, intestinal deficiency of Rev-erbα, a known BMAL1 repressor, enhances dietary fat absorption and exacerbates HFD-induced obesity and comorbidities. Moreover, small-molecule targeting of REV-ERBα/BMAL1 by SR9009 ameliorates HFD-induced obesity in mice. Altogether, intestine clock functions as an accelerator in dietary fat absorption and targeting intestinal BMAL1 may be a promising approach for management of metabolic diseases induced by excess fat intake.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Circadian Rhythm / Diacylglycerol O-Acyltransferase / Fatty Liver / ARNTL Transcription Factors / Nuclear Receptor Subfamily 1, Group D, Member 1 / Hyperlipidemias / Obesity Type of study: Etiology_studies Language: En Journal: Nat Commun Journal subject: BIOLOGIA / CIENCIA Year: 2021 Document type: Article Affiliation country: China

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Circadian Rhythm / Diacylglycerol O-Acyltransferase / Fatty Liver / ARNTL Transcription Factors / Nuclear Receptor Subfamily 1, Group D, Member 1 / Hyperlipidemias / Obesity Type of study: Etiology_studies Language: En Journal: Nat Commun Journal subject: BIOLOGIA / CIENCIA Year: 2021 Document type: Article Affiliation country: China