Your browser doesn't support javascript.
loading
Characterization of Oligomeric Proanthocyanidin-Enriched Fractions from Aronia melanocarpa (Michx.) Elliott via High-Resolution Mass Spectrometry and Investigations on Their Inhibitory Potential on Human Topoisomerases.
Müller, Lena; Weever, Fabian; Hübner, Florian; Humpf, Hans-Ulrich; Esselen, Melanie.
Affiliation
  • Müller L; Institute of Food Chemistry, University of Muenster, Corrensstraße 45, Münster 48149, Germany.
  • Weever F; Institute of Food Chemistry, University of Muenster, Corrensstraße 45, Münster 48149, Germany.
  • Hübner F; Institute of Food Chemistry, University of Muenster, Corrensstraße 45, Münster 48149, Germany.
  • Humpf HU; Institute of Food Chemistry, University of Muenster, Corrensstraße 45, Münster 48149, Germany.
  • Esselen M; Institute of Food Chemistry, University of Muenster, Corrensstraße 45, Münster 48149, Germany.
J Agric Food Chem ; 69(37): 11053-11064, 2021 Sep 22.
Article in En | MEDLINE | ID: mdl-34495660
Aronia melanocarpa (MICHX.) ELLIOTT, which belongs to the Rosaceae family, has increasingly come into focus of research due to the high content of polyphenols. In addition to antioxidative properties, further health-promoting effects of these polyphenols are still of interest. Especially, the proanthocyanidins offer thereby huge opportunities due to their high structural heterogeneity. Therefore, the present study focuses on the topoisomerase inhibiting effects of oligomeric proanthocyanidins (PACs), which are potentially depended on their degree of polymerization. The investigated PACs isolated from Aronia berries were characterized by chromatographic techniques and liquid chromatography-high-resolution mass spectrometry. Four PAC enriched fractions were obtained from Aronia pomace containing 47 PACs with a degree of polymerization from three to six. Due to the low yield of hexamers, the potential inhibiting effects against human topoisomerase were investigated for the trimer to pentamer fractions. The relaxation and decatenation assays were performed to examine the inhibiting effect on topoisomerases under cell-free conditions. Moreover, rapid isolation of topoisomerase cleavage complexes in human colon carcinoma HT29 cells was performed to evaluate the effect on topoisomerases in a cell-based system. The fractions demonstrated inhibitory potential on topoisomerases I and II. In sum, an increasing effect strength depending on the degree of polymerization was shown.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rosaceae / Proanthocyanidins / Photinia Limits: Humans Language: En Journal: J Agric Food Chem Year: 2021 Document type: Article Affiliation country: Germany Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rosaceae / Proanthocyanidins / Photinia Limits: Humans Language: En Journal: J Agric Food Chem Year: 2021 Document type: Article Affiliation country: Germany Country of publication: United States