Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli.

Marivate, Amukelani; Njengele-Tetyana, Zikhona; Fish, Muhammad Qasim; Mosebi, Salerwe

Marivate, Amukelani; Njengele-Tetyana, Zikhona; Fish, Muhammad Qasim; Mosebi, Salerwe.

Affiliation

Marivate A; Biomedical Group, Advanced Materials Division, Mintek, Randburg, 2125, South Africa. Electronic address: amukelanim@mintek.co.za.
Njengele-Tetyana Z; Biomedical Group, Advanced Materials Division, Mintek, Randburg, 2125, South Africa.
Fish MQ; Biomedical Group, Advanced Materials Division, Mintek, Randburg, 2125, South Africa.
Mosebi S; Department of Life and Consumer Sciences, College of Agriculture and Environmental Sciences, University of South Africa, Private Bag X6, Florida, 1710, South Africa.

Protein Expr Purif ; 188: 105969, 2021 12.

Article in En | MEDLINE | ID: mdl-34500069

Subject(s)

Antigens, CD/genetics; HIV-1/drug effects; Host-Pathogen Interactions/genetics; Human Immunodeficiency Virus Proteins/metabolism; Viral Regulatory and Accessory Proteins/metabolism; Viroporin Proteins/metabolism; Amino Acid Sequence; Antigens, CD/biosynthesis; Antigens, CD/isolation & purification; Antigens, CD/pharmacology; Base Sequence; Chromatography, Affinity/methods; Chromatography, Ion Exchange/methods; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/metabolism; GPI-Linked Proteins/biosynthesis; GPI-Linked Proteins/genetics; GPI-Linked Proteins/isolation & purification; GPI-Linked Proteins/pharmacology; Gene Expression; Genetic Vectors/chemistry; Genetic Vectors/metabolism; HIV Infections/drug therapy; HIV Infections/virology; HIV-1/genetics; HIV-1/metabolism; HIV-1/pathogenicity; Human Immunodeficiency Virus Proteins/genetics; Humans; Inclusion Bodies/chemistry; Protein Binding; Protein Refolding; Recombinant Proteins/biosynthesis; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Recombinant Proteins/pharmacology; Viral Regulatory and Accessory Proteins/genetics; Viroporin Proteins/genetics

Key words

BST-2; HIV-1; Innate immunity; Membrane protein; Over-expression; Purification; Renaturation

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Antigens, CD / HIV-1 / Human Immunodeficiency Virus Proteins / Viral Regulatory and Accessory Proteins / Host-Pathogen Interactions / Viroporin Proteins Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 2021 Document type: Article Country of publication: United States

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