Extended disorder at the cell surface: The conformational landscape of the ectodomains of syndecans.

Gondelaud, Frank; Bouakil, Mathilde; Le Fèvre, Aurélien; Miele, Adriana Erica; Chirot, Fabien; Duclos, Bertrand; Liwo, Adam; Ricard-Blum, Sylvie

Gondelaud, Frank; Bouakil, Mathilde; Le Fèvre, Aurélien; Miele, Adriana Erica; Chirot, Fabien; Duclos, Bertrand; Liwo, Adam; Ricard-Blum, Sylvie.

Affiliation

Gondelaud F; Univ Lyon, University Claude Bernard Lyon 1, CNRS, INSA Lyon, CPE, Institute of Molecular and Supramolecular Chemistry and Biochemistry (ICBMS), UMR 5246, F-69622 Villeurbanne cedex, France.
Bouakil M; Univ Lyon, University Claude Bernard Lyon 1, CNRS, Institut Lumière Matière, UMR 5306, Cité Lyonnaise de l'Environnement et de l'Analyse, 5 rue de la Doua, 69100 Villeurbanne, France.
Le Fèvre A; Univ Lyon, University Claude Bernard Lyon 1, CNRS, Institut des Sciences Analytiques, UMR 5280, 5 Rue de la Doua, 69100 Villeurbanne, France.
Miele AE; Univ Lyon, University Claude Bernard Lyon 1, CNRS, INSA Lyon, CPE, Institute of Molecular and Supramolecular Chemistry and Biochemistry (ICBMS), UMR 5246, F-69622 Villeurbanne cedex, France.
Chirot F; Univ Lyon, University Claude Bernard Lyon 1, CNRS, Institut des Sciences Analytiques, UMR 5280, 5 Rue de la Doua, 69100 Villeurbanne, France.
Duclos B; Univ Lyon, University Claude Bernard Lyon 1, CNRS, INSA Lyon, CPE, Institute of Molecular and Supramolecular Chemistry and Biochemistry (ICBMS), UMR 5246, F-69622 Villeurbanne cedex, France.
Liwo A; Laboratory of Molecular Modeling, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland.
Ricard-Blum S; Univ Lyon, University Claude Bernard Lyon 1, CNRS, INSA Lyon, CPE, Institute of Molecular and Supramolecular Chemistry and Biochemistry (ICBMS), UMR 5246, F-69622 Villeurbanne cedex, France.

Matrix Biol Plus ; 12: 100081, 2021 Dec.

Article in En | MEDLINE | ID: mdl-34505054

Key words

CCS, collision cross section; CD, circular dichroism; CSD, charge state distribution; Cell-matrix interactions; Conformations; DLS, dynamic light scattering; DTT, dithiothreitol; ED, ectodomain; ESI-IM-MS, electrospray ionization - ion mobility - mass spectrometry; ESI-MS, electrospray ionization - mass spectrometry; GAG, glycosaminoglycan; IDP, intrinsically disordered protein; Intrinsically disordered proteins; MoRF, molecular recognition feature; PAGE, polyacrylamide gel electrophoresis; PMG, pre-molten globule; RC, random-coil; SASA, solvent accessible surface area; SAXS, small angle X-ray scattering; SDC, syndecan; SDS, sodium dodecyl sulfate; SEC, size exclusion chromatography; Syndecans; TFE, trifluoroethanol

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Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Prognostic_studies Language: En Journal: Matrix Biol Plus Year: 2021 Document type: Article Affiliation country: France Country of publication: Netherlands

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