Background-free sensing platform for on-site detection of carbamate pesticide through upconversion nanoparticles-based hydrogel suit.
Biosens Bioelectron
; 194: 113598, 2021 Dec 15.
Article
in En
| MEDLINE
| ID: mdl-34507097
ABSTRACT
On-site monitoring of carbamate pesticide in complex matrix remians as a challenge in terms of the real-time control of food safety and supervision of environmental quality. Herein, we fabricated robust upconversion nanoparticles (UCNPS)/polydopamine (PDA)-based hydrogel portable suit that precisely quantified carbaryl in complex tea samples with smartphone detector. UCNPS/PDA nanoprobe was developed by polymerization of dopamine monomers on the surface of NaErF4 0.5% Tm3+@NaYF4 through electrostatic interaction, leading to efficient red luminescence quenching of UCNPS under near-infrared excitation, which circumvented autofluorescence and background interference in complicated environment. Such a luminescence quenching could be suppressed by thiocholine that was produced by acetylcholinesterase-mediated catalytic reaction, thus enabling carbaryl bioassay by inhibiting the activity of enzyme. Bestowed with the feasibility analysis of fluorescent output, portable platform was designed by integrating UCNPS-embedded sodium alginate hydrogel with 3D-printed smartphone device for quantitatively on-site monitoring of carbaryl in the range of 0.5-200 ng mL-1 in tea sample, accompanied by a detection limit of 0.5 ng mL-1. Owing to specific UCNPS signatures and hydrogel immobilization, this modular platform displayed sensitive response, portability and anti-interference capability in complex matrix analysis, thus holding great potential in point-of-care application.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pesticides
/
Biosensing Techniques
/
Nanoparticles
Type of study:
Diagnostic_studies
Language:
En
Journal:
Biosens Bioelectron
Journal subject:
BIOTECNOLOGIA
Year:
2021
Document type:
Article