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A gain series method for accurate EMCCD calibration.
Ryan, Duncan P; Dunlap, Megan K; Gelfand, Martin P; Werner, James H; Van Orden, Alan K; Goodwin, Peter M.
Affiliation
  • Ryan DP; Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, 87545, USA. ryand@lanl.gov.
  • Dunlap MK; Department of Chemistry, Colorado State University, Fort Collins, CO, 80523, USA.
  • Gelfand MP; Department of Physics, Colorado State University, Fort Collins, CO, 80523, USA.
  • Werner JH; Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, 87545, USA.
  • Van Orden AK; Department of Chemistry, Colorado State University, Fort Collins, CO, 80523, USA.
  • Goodwin PM; Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, 87545, USA.
Sci Rep ; 11(1): 18348, 2021 Sep 15.
Article in En | MEDLINE | ID: mdl-34526588
ABSTRACT
Calibration of the gain and digital conversion factor of an EMCCD is necessary for accurate photon counting. We present a new method to quickly calibrate multiple gain settings of an EMCCD camera. Acquiring gain-series calibration data and analyzing the resulting images with the EMCCD noise model more accurately estimates the gain response of the camera. Furthermore, we develop a method to compare the results from different calibration approaches. Gain-series calibration outperforms all other methods in this self-consistency test.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Prognostic_studies Language: En Journal: Sci Rep Year: 2021 Document type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Prognostic_studies Language: En Journal: Sci Rep Year: 2021 Document type: Article Affiliation country: United States