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MicroRNA Dysregulation in the Hippocampus of Rats with Noise-Induced Hearing Loss.
Ha, Seungmin; Kim, Kyung Woon; Lee, So Min; Lee, Chang Ho; Kim, So Young.
Affiliation
  • Ha S; Department of Otorhinolaryngology Head & Neck Surgery, CHA University College of Medicine, Bundang CHA Medical Center, Republic of Korea.
  • Kim KW; Department of Otorhinolaryngology Head & Neck Surgery, CHA University College of Medicine, Bundang CHA Medical Center, Republic of Korea.
  • Lee SM; Department of Otorhinolaryngology Head & Neck Surgery, CHA University College of Medicine, Bundang CHA Medical Center, Republic of Korea.
  • Lee CH; Department of Otorhinolaryngology Head & Neck Surgery, CHA University College of Medicine, Bundang CHA Medical Center, Republic of Korea.
  • Kim SY; Department of Otorhinolaryngology Head & Neck Surgery, CHA University College of Medicine, Bundang CHA Medical Center, Republic of Korea.
Oxid Med Cell Longev ; 2021: 1377195, 2021.
Article in En | MEDLINE | ID: mdl-34527169
ABSTRACT
Although hippocampal changes due to noise-induced hearing loss have been suggested, little is known about the miRNA levels due to these hippocampal changes. Three-week-old Sprague-Dawley rats were divided into noise and control groups (n = 20 per group). The noise group rats were exposed to white Gaussian noise (115 dB SPL, 4 hours per day) for three days. One day after noise exposure, the hippocampi of rats were harvested and miRNA expressions were analyzed using the Affymetrix miRNA 4.0 microarray (n = 6 per group). The predicted target genes of each miRNA were retrieved, and the pathways related to the predicted target genes were analyzed. miR-758-5p, miR-210-5p, miR-370-5p, miR-652-5p, miR-3544, miR-128-1-5p, miR-665, miR-188-5p, and miR-874-5p expression increased in the hippocampal tissue of the noise group compared to that in the control group. The overlapping predicted target genes included Bend4, Creb1, Adcy6, Creb5, Kcnj9, and Pten. The pathways related to these genes were the estrogen signaling pathway, vasopressin-regulated water reabsorption, thyroid hormone synthesis, aldosterone synthesis and secretion, insulin secretion, circadian entrainment, insulin resistance, cholinergic synapse, dopaminergic synapse, cGMP-PKG signaling pathway, cAMP signaling pathway, PI3K-Akt signaling pathway, TNF signaling pathway, and AMPK signaling pathway. miR-448-3p, miR204-5p, and miR-204-3p expression decreased in the hippocampal tissue of the noise group compared to that in the control group. The overlapping predicted target genes of these three miRNAs were Rps6kas, Nfactc3, Rictor, Spred1, Cdh4, Cdh6, Dvl3, and Rcyt1b. Pathway analysis suggested that the Wnt signaling pathway is related to Dvl3 and Nfactc3. Noise-induced hearing loss dysregulates miR-758-5p, miR210-5p, miR370-5p, miR-652-5p, miR-3544, miR-128-1-5p, miR-665, miR-188-5p, miR-874-5p, miR-448-3p, miR-204-5p, miR-204-3p, and miR-140-5p expression in the hippocampus. These miRNAs have been predicted to be associated with hormonal, inflammatory, and synaptic pathways.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: MicroRNAs / Hearing Loss, Noise-Induced / Hippocampus Type of study: Prognostic_studies Limits: Animals Language: En Journal: Oxid Med Cell Longev Journal subject: METABOLISMO Year: 2021 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: MicroRNAs / Hearing Loss, Noise-Induced / Hippocampus Type of study: Prognostic_studies Limits: Animals Language: En Journal: Oxid Med Cell Longev Journal subject: METABOLISMO Year: 2021 Document type: Article