Purification of GFP-tagged nuclei from frozen livers of INTACT mice for RNA- and ATAC-sequencing.

Loft, Anne; Herzig, Stephan; Schmidt, Søren Fisker

Loft, Anne; Herzig, Stephan; Schmidt, Søren Fisker.

Affiliation

Loft A; Institute for Diabetes and Cancer (IDC), Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg 85764, Germany.
Herzig S; Joint Heidelberg-IDC Translational Diabetes Program, Heidelberg University Hospital, Heidelberg 69120, Germany.
Schmidt SF; Molecular Metabolic Control, Medical Faculty, Technical University Munich, München 80333, Germany.

STAR Protoc ; 2(3): 100805, 2021 09 17.

Article in En | MEDLINE | ID: mdl-34527961

Subject(s)

Cell Nucleus/chemistry; Chromatin Immunoprecipitation Sequencing; Cytological Techniques/methods; Green Fluorescent Proteins/chemistry; Liver/cytology; RNA-Seq; Animals; Cell Nucleus/metabolism; Female; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Liver/chemistry; Male; Mice

Key words

Cell Biology; Cell isolation; Cell separation/fractionation; Genomics; Molecular Biology; RNAseq; Sequencing

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Nucleus / Cytological Techniques / Green Fluorescent Proteins / Chromatin Immunoprecipitation Sequencing / RNA-Seq / Liver Limits: Animals Language: En Journal: STAR Protoc Year: 2021 Document type: Article Affiliation country: Germany

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