Transcriptome and Methylome Analysis Reveal Complex Cross-Talks between Thyroid Hormone and Glucocorticoid Signaling at Xenopus Metamorphosis.
Cells
; 10(9)2021 09 09.
Article
in En
| MEDLINE
| ID: mdl-34572025
ABSTRACT
BACKGROUND:
Most work in endocrinology focus on the action of a single hormone, and very little on the cross-talks between two hormones. Here we characterize the nature of interactions between thyroid hormone and glucocorticoid signaling during Xenopus tropicalis metamorphosis.METHODS:
We used functional genomics to derive genome wide profiles of methylated DNA and measured changes of gene expression after hormonal treatments of a highly responsive tissue, tailfin. Clustering classified the data into four types of biological responses, and biological networks were modeled by system biology.RESULTS:
We found that gene expression is mostly regulated by either T3 or CORT, or their additive effect when they both regulate the same genes. A small but non-negligible fraction of genes (12%) displayed non-trivial regulations indicative of complex interactions between the signaling pathways. Strikingly, DNA methylation changes display the opposite and are dominated by cross-talks.CONCLUSION:
Cross-talks between thyroid hormones and glucocorticoids are more complex than initially envisioned and are not limited to the simple addition of their individual effects, a statement that can be summarized with the pseudo-equation TH â GC > TH + GC. DNA methylation changes are highly dynamic and buffered from genome expression.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Thyroid Hormones
/
Xenopus
/
Signal Transduction
/
Transcriptome
/
Glucocorticoids
/
Metamorphosis, Biological
Limits:
Animals
Language:
En
Journal:
Cells
Year:
2021
Document type:
Article
Affiliation country:
France