Variation and Selection in the Putative Sperm-Binding Region of ZP3 in Muroid Rodents: A Comparison between Cricetids and Murines.
Genes (Basel)
; 12(9)2021 09 20.
Article
in En
| MEDLINE
| ID: mdl-34573431
ABSTRACT
In mammals, the zona pellucida glycoprotein 3 (ZP3) is considered a primary sperm receptor of the oocyte and is hypothesized to be involved in reproductive isolation. We investigated patterns of diversity and selection in the putative sperm-binding region (pSBR) of mouse ZP3 across Cricetidae and Murinae, two hyperdiverse taxonomic groups within muroid rodents. In murines, the pSBR is fairly conserved, in particular the serine-rich stretch containing the glycosylation sites proposed as essential for sperm binding. In contrast, cricetid amino acid sequences of the pSBR were much more variable and the serine-rich motif, typical of murines, was generally substantially modified. Overall, our results suggest a general lack of species specificity of the pSBR across the two muroid families. We document statistical evidence of positive selection acting on exons 6 and 7 of ZP3 and identified several amino acid sites that are likely targets of selection, with most positively selected sites falling within or adjacent to the pSBR.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arvicolinae
/
Murinae
/
Zona Pellucida Glycoproteins
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Genes (Basel)
Year:
2021
Document type:
Article
Affiliation country:
Portugal