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Specific deletion of protein phosphatase 6 catalytic subunit in Sertoli cells leads to disruption of spermatogenesis.
Lei, Wen-Long; Li, Yuan-Yuan; Meng, Tie-Gang; Ning, Yan; Sun, Si-Min; Zhang, Chun-Hui; Gui, Yaoting; Wang, Zhen-Bo; Qian, Wei-Ping; Sun, Qing-Yuan.
Affiliation
  • Lei WL; Department of Reproductive Medicine, Peking University Shenzhen Hospital, Shenzhen, 518036, China.
  • Li YY; CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
  • Meng TG; Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen, 518036, China.
  • Ning Y; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
  • Sun SM; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
  • Zhang CH; Fertility Preservation Lab, Guangdong-Hong Kong Metabolism & Reproduction Joint Laboratory, Reproductive Medicine Center, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China.
  • Gui Y; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
  • Wang ZB; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
  • Qian WP; Department of Reproductive Medicine, Peking University Shenzhen Hospital, Shenzhen, 518036, China.
  • Sun QY; Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen, 518036, China.
Cell Death Dis ; 12(10): 883, 2021 09 27.
Article in En | MEDLINE | ID: mdl-34580275
Protein phosphatase 6 (PP6) is a member of the PP2A-like subfamily, which plays significant roles in numerous fundamental biological activities. We found that PPP6C plays important roles in male germ cells recently. Spermatogenesis is supported by the Sertoli cells in the seminiferous epithelium. In this study, we crossed Ppp6cF/F mice with AMH-Cre mice to gain mutant mice with specific depletion of the Ppp6c gene in the Sertoli cells. We discovered that the PPP6C cKO male mice were absolutely infertile and germ cells were largely lost during spermatogenesis. By combing phosphoproteome with bioinformatics analysis, we showed that the phosphorylation status of ß-catenin at S552 (a marker of adherens junctions) was significantly upregulated in mutant mice. Abnormal ß-catenin accumulation resulted in impaired testicular junction integrity, thus led to abnormal structure and functions of BTB. Taken together, our study reveals a novel function for PPP6C in male germ cell survival and differentiation by regulating the cell-cell communication through dephosphorylating ß-catenin at S552.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sertoli Cells / Spermatogenesis / Gene Deletion / Phosphoprotein Phosphatases / Catalytic Domain Limits: Animals Language: En Journal: Cell Death Dis Year: 2021 Document type: Article Affiliation country: China Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sertoli Cells / Spermatogenesis / Gene Deletion / Phosphoprotein Phosphatases / Catalytic Domain Limits: Animals Language: En Journal: Cell Death Dis Year: 2021 Document type: Article Affiliation country: China Country of publication: United kingdom