Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq.
STAR Protoc
; 2(4): 100842, 2021 12 17.
Article
in En
| MEDLINE
| ID: mdl-34585169
ABSTRACT
Here, we outline detailed protocols to isolate and profile murine splenic dendritic cells (DCs) through advanced flow cytometry of the myeloid compartment and single-cell transcriptomic profiling with integrated cell surface protein expression through CITE-seq. This protocol provides a general transferrable road map for different tissues and species. For complete details on the use and execution of this protocol, please refer to Lukowski et al. (2021).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Gene Expression Profiling
/
Myeloid Cells
Limits:
Animals
Language:
En
Journal:
STAR Protoc
Year:
2021
Document type:
Article
Affiliation country:
Sweden