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Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq.
Rødahl, Inga; Gotley, James; Andersen, Stacey B; Yu, Meihua; Mehdi, Ahmed M; Christ, Angelika N; Hamilton-Williams, Emma E; Frazer, Ian H; Lukowski, Samuel W; Chandra, Janin.
Affiliation
  • Rødahl I; Center for Infectious Medicine, Department of Medicine, Karolinska Institute, Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden.
  • Gotley J; The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia.
  • Andersen SB; The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia.
  • Yu M; The Institute for Molecular Bioscience, The University of Queensland, Woolloongabba, QLD 4067, Australia.
  • Mehdi AM; The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia.
  • Christ AN; The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia.
  • Hamilton-Williams EE; The Institute for Molecular Bioscience, The University of Queensland, Woolloongabba, QLD 4067, Australia.
  • Frazer IH; The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia.
  • Lukowski SW; The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia.
  • Chandra J; The Institute for Molecular Bioscience, The University of Queensland, Woolloongabba, QLD 4067, Australia.
STAR Protoc ; 2(4): 100842, 2021 12 17.
Article in En | MEDLINE | ID: mdl-34585169
ABSTRACT
Here, we outline detailed protocols to isolate and profile murine splenic dendritic cells (DCs) through advanced flow cytometry of the myeloid compartment and single-cell transcriptomic profiling with integrated cell surface protein expression through CITE-seq. This protocol provides a general transferrable road map for different tissues and species. For complete details on the use and execution of this protocol, please refer to Lukowski et al. (2021).
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Gene Expression Profiling / Myeloid Cells Limits: Animals Language: En Journal: STAR Protoc Year: 2021 Document type: Article Affiliation country: Sweden
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Gene Expression Profiling / Myeloid Cells Limits: Animals Language: En Journal: STAR Protoc Year: 2021 Document type: Article Affiliation country: Sweden