Detection of Major Human Herpesviruses in Iranian Patients with Suspected Encephalitis Using Multiplex Polymerase Chain Reaction.
Eur Neurol
; 85(2): 142-147, 2022.
Article
in En
| MEDLINE
| ID: mdl-34587615
ABSTRACT
BACKGROUND:
The Herpesviridae family plays a significant etiological role in central nervous system viral infections during primary infection or reactivation from a latent form. Early detection is crucial because prescribing some antivirals can prevent severe side effects or life-threatening conditions.METHODS:
In this study, 251 CSF specimens were collected from patients with clinical suspicion of viral encephalitis in Pars Hospital, Tehran, Iran. DNA was extracted, and a multiplex PCR was designed to investigate the presence of herpes simplex virus-1, herpes simplex virus-2, varicella zoster virus, Epstein-Barr virus, and cytomegalovirus.RESULTS:
Overall, 59 cases of the 251 CSF samples were positive for multiplex PCR (23.5%). The most frequent positive findings were EBV and HSV, with a prevalence of 10.3% and 8.7% (5.5% HSV-1 and 3.1% HSV-2), respectively. Four co-infections were also seen in this study.CONCLUSIONS:
This multiplex PCR assay detects simultaneously different herpesviruses in CSF samples of patients with suspected encephalitis in 2 rounds of PCR amplification; therefore, it is a reliable and cost-saving diagnostic method for evaluating patients infected with herpesvirus with neurological disorders.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Herpesviridae Infections
/
Epstein-Barr Virus Infections
/
Encephalitis
Type of study:
Diagnostic_studies
/
Risk_factors_studies
/
Screening_studies
Limits:
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
Eur Neurol
Year:
2022
Document type:
Article
Affiliation country:
Iran