A Genetic Interaction Screening Approach in C. elegans.
Methods Mol Biol
; 2381: 79-95, 2021.
Article
in En
| MEDLINE
| ID: mdl-34590271
ABSTRACT
Genetic interaction screens have played a critical role in better understanding epistasis and functional relationships among genes. These screens have been conducted at multiple scales, ranging from testing pairwise interactions genome-wide in yeast and bacteria, to more focused screens in multicellular organisms and cultured cells. Here, I describe a strategy that facilitates genetic interaction screens with loss of function alleles in the model organism Caenorhabditis elegans. I also present a simple downstream assay to measure the effects of combinations of mutations on fitness.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Caenorhabditis elegans
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Animals
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2021
Document type:
Article
Affiliation country:
Canada