Disinfection and conditions associated with thermo-assisted drying and decontamination inconsistently produce negative PRRSV rRT-PCR results on metal surfaces.
Vet Microbiol
; 262: 109240, 2021 Nov.
Article
in En
| MEDLINE
| ID: mdl-34600200
ABSTRACT
Because contaminated livestock trailers are a significant risk for transmitting viruses between herds, various methods of washing, disinfecting, and thermo-assisted drying and decontamination (TADD) have been evaluated for their effectiveness in inactivating porcine reproductive and respiratory syndrome virus (PRRSV) on contaminated surfaces. Information on when to expect negative qRT-PCR results after adequate trailer sanitation is lacking. The objective of this study was to evaluate whether there are conditions associated with washing-disinfectant-TADD procedures that will consistently produce a negative qRT-PCR result for the purpose of monitoring compliance with trailer sanitation and decontamination protocols for PRRSV on metal surfaces. 144 diamond plate aluminum coupons were spiked with PRRSV or phosphate-buffered saline (PBS) and treated with a designated disinfectant protocol. Disinfectants evaluated included multiple accelerated® hydrogen peroxide (AHP) disinfectants and a quaternary ammonium and glutaraldehyde combination disinfectant. Disinfectant was applied for 5 or 60 minutes of contact time at either 20 °C or -10 °C in a matrix of feces or PBS. All coupons were heated until the surface temperature of the coupon reached 71 °C and then held for 10 minutes to simulate TADD under field conditions. Post-treatment swabs for all treatment groups, except negative control groups, were positive by PRRSV qRT-PCR. Under the conditions evaluated in this study, consistently negative qRT-PCR results after treatments were not found. Therefore, for the purpose of monitoring compliance with trailer sanitation and decontamination protocols for PRRSV, alternatives to qRT-PCR should be explored.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Industrial Microbiology
/
Decontamination
/
Disinfection
/
Porcine respiratory and reproductive syndrome virus
/
Reverse Transcriptase Polymerase Chain Reaction
/
Metals
Type of study:
Guideline
/
Risk_factors_studies
Limits:
Animals
Language:
En
Journal:
Vet Microbiol
Year:
2021
Document type:
Article
Publication country:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS