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Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples.
Rifflet, Aline; Demangel, Caroline; Guenin-Macé, Laure.
Affiliation
  • Rifflet A; Biology and Genetics of Bacterial Cell Wall, Institut Pasteur, Paris, France.
  • Demangel C; INSERM, Equipe Avenir, Paris, France.
  • Guenin-Macé L; Immunobiology of Infection Unit, Institut Pasteur, INSERM U1221, Paris, France.
Methods Mol Biol ; 2387: 117-130, 2022.
Article in En | MEDLINE | ID: mdl-34643908
ABSTRACT
Mycolactones are a family of polyketide synthase products made by the human pathogen Mycobacterium ulcerans that were recently identified as novel inhibitors of the host membrane translocation complex (Sec61). Here, we provide protocols for the purification of mycolactones from bacterial cultures, and for their quantitative assessment in biological samples.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chromatography, High Pressure Liquid Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2022 Document type: Article Affiliation country: France
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chromatography, High Pressure Liquid Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2022 Document type: Article Affiliation country: France