Elevated Foxo3a and Fas-ligand expression in human periapical granulomas as a potential treatment target.
Oral Dis
; 29(3): 1128-1136, 2023 Apr.
Article
in En
| MEDLINE
| ID: mdl-34674361
ABSTRACT
OBJECTIVE:
Periapical granuloma is a common periodontitis type involving chronic inflammation; however, the efficacy of current therapies is limited. Its molecular pathogenesis also remains obscure. Forkhead box transcription factor class o3a (Foxo3a) and Fas-ligand (FasL) are associated with chronic inflammation. Therefore, in this study, we aimed to clarify the roles of Foxo3a and FasL in periapical granuloma pathophysiology. SUBJECTS ANDMETHODS:
Periapical lesions were obtained from patients during endodontic surgery and tooth extraction; those diagnosed with periapical granulomas using haematoxylin and eosin staining were further analysed. Immunohistochemical analysis was performed for Foxo3a and FasL, and real-time polymerase chain reaction was performed for FOXO3A, FASL and interleukin (IL)-1ß. Healthy gingival tissues were also examined as controls.RESULTS:
Neutrophils, lymphocytes and plasma cells in the periapical granulomas, but not healthy tissues, expressed Foxo3a. Dual-colour immunofluorescence imaging revealed Foxo3a and FasL co-expression in leukocytes. FOXO3A, FASL and IL-1ß mRNA levels in healthy gingival tissues were significantly lower than those in the periapical granulomas. Additionally, FOXO3A and IL-1ß expressions were negatively correlated.CONCLUSIONS:
Phosphorylated Foxo3a may reduce IL-1ß release by inhibiting apoptosis through FasL in periapical periodontitis and prevent exacerbation. Thus, Foxo3a is a potential therapeutic agent for periapical periodontitis.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Periapical Granuloma
/
Periapical Periodontitis
Limits:
Humans
Language:
En
Journal:
Oral Dis
Journal subject:
ODONTOLOGIA
Year:
2023
Document type:
Article
Affiliation country:
Japan
Publication country:
DENMARK
/
DINAMARCA
/
DK