Construction of Genomic and Sub-Genomic Dengue Infectious Replicons.
Methods Mol Biol
; 2409: 77-96, 2022.
Article
in En
| MEDLINE
| ID: mdl-34709637
ABSTRACT
Dengue replicons are powerful tools used in studying virus biology as well as in high-throughput screening of drug candidates. Replicon constructs are developed as genomic (consists of all the viral protein genes) or sub-genomic (consists of only nonstructural protein genes) and are used to study various aspects of the virus life cycle such as genome replication and virus assembly. In addition, a replicon usually includes a reporter gene used in monitoring virus replication. In this chapter, we provide methods to develop both genomic and sub-genomic dengue replicons with a luciferase reporter and describe different assays to utilize these systems.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Dengue Virus
Limits:
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2022
Document type:
Article
Affiliation country:
United States