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The yellowhorn AGL transcription factor gene XsAGL22 contributes to ABA biosynthesis and drought tolerance in poplar.
Bian, Zhan; Wang, Xiaoling; Lu, Junkun; Wang, Dongli; Zhou, Yangyan; Liu, Yunshan; Wang, Shengkun; Yu, Zequn; Xu, Daping; Meng, Sen.
Affiliation
  • Bian Z; State Key Laboratory of Tree Genetics and Breeding, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, Guangdong 510520, China.
  • Wang X; Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang, Jiangxi 330096, China.
  • Lu J; State Key Laboratory of Tree Genetics and Breeding, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, Guangdong 510520, China.
  • Wang D; State Key Laboratory of Tree Genetics and Breeding, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, Guangdong 510520, China.
  • Zhou Y; Salver Academy of Botany, Rizhao, Shandong 262300, China.
  • Liu Y; College of Biology and Food Engineering, Chongqing Three Gorges University, Wanzhou, Chongqing 404100, China.
  • Wang S; State Key Laboratory of Tree Genetics and Breeding, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, Guangdong 510520, China.
  • Yu Z; Shanghai Gardening-Landscaping Construction Co., Ltd, Shanghai 200333, China.
  • Xu D; State Key Laboratory of Tree Genetics and Breeding, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, Guangdong 510520, China.
  • Meng S; State Key Laboratory of Tree Genetics and Breeding, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, Guangdong 510520, China.
Tree Physiol ; 42(6): 1296-1309, 2022 06 09.
Article in En | MEDLINE | ID: mdl-34726236
ABSTRACT
Regulation of abscisic acid (ABA) biosynthesis helps plants adapt to drought stress, but the underlying molecular mechanisms are largely unclear. Here, a drought-induced transcription factor XsAGL22 was isolated from yellowhorn (Xanthoceras sorbifolium Bunge). Yeast one-hybrid and electrophoretic mobility shift assays indicated that XsAGL22 can physically bind to the promoters of the ABA biosynthesis-related genes XsNCED6 and XsBG1, and a dual-luciferase assay showed that XsAGL22 activates the promoters of the later two genes. Transient overexpression of XsAGL22 in yellowhorn leaves also increased the expression of XsNCED6 and XsBG1 and increased cellular ABA levels. Finally, heterologous overexpression of XsAGL22 in poplar increased ABA content, reduced stomatal aperture and increased drought resistance. Our results suggest that XsAGL22 is a powerful regulator of ABA biosynthesis and plays a critical role in drought resistance in plants.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Populus / Droughts Language: En Journal: Tree Physiol Journal subject: BOTANICA / FISIOLOGIA Year: 2022 Document type: Article Affiliation country: China
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Populus / Droughts Language: En Journal: Tree Physiol Journal subject: BOTANICA / FISIOLOGIA Year: 2022 Document type: Article Affiliation country: China