Effective definition of low humoral response to Clostridioides difficile infection.
Anaerobe
; 72: 102475, 2021 Dec.
Article
in En
| MEDLINE
| ID: mdl-34752901
ABSTRACT
BACKGROUND:
Determination of the humoral response to Clostridioides difficile (CD) toxins could be of great value in the management of patients with CD infection (CDI).METHODS:
A prospective study was conducted on the clinical characteristics and humoral response in patients with CDI. Determination of ELISA IgG CD anti-toxin B (tgcBiomics, Germany) was performed. The following dilutions were planned for each patient, 1100, 1 200, 1 400, 1 800 1 1600. A significant concentration of antibody was considered to be present in each dilution if an optical density 0.2 units higher than the negative control of the technique was evident.RESULTS:
Eighty-five patients were included during the study period, November 2018-February 2020. The median age was 73 years (interquartile range 62.5-85 years), with female predominance (45 patients, 52.9%). Thirty-nine patients (45.9%) had a severe infection. Seven patients (8.2%) had suffered an episode of CDI in the previous three months. Seventeen patients (20%) had one or more recurrent episodes during the three-month follow-up No patient died during admission or required surgery for severe-complicated infection. The incidence of recurrence in patients with no antibody detected at 1400 dilution was 25.4% (16 patients) while it was 4.3% (one patient) in patients with antibody present at that dilution (p = 0.03). Liver cirrhosis was associated with higher humoral response against CD.CONCLUSIONS:
Antibodies IgG CD anti-toxin B detection at a dilution of 1400, using a B ELISA technique, effectively identified patients at increased risk of recurrence. This information could help assist in the management of patients.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Clostridioides difficile
/
Clostridium Infections
/
Host-Pathogen Interactions
/
Immunity, Humoral
Type of study:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
Country/Region as subject:
Europa
Language:
En
Journal:
Anaerobe
Year:
2021
Document type:
Article