Simultaneous disruption of circulating miR-21 and cytotoxic T lymphocytes (CTLs): Prospective diagnostic and prognostic markers for esophageal squamous cell carcinoma (ESCC).
J Clin Lab Anal
; 36(1): e24125, 2022 Jan.
Article
in En
| MEDLINE
| ID: mdl-34799871
ABSTRACT
BACKGROUND:
Esophageal squamous cell carcinoma (ESCC) as the most prominent type of esophageal cancer (EC) in developing countries encompasses a substantial contribution of cancer-related mortalities and morbidities. Cytotoxic T lymphocytes (CTLs) are the major subset of effector T cells against cancer. However, the microRNAs involved in the development and regulation of CTLs could be disrupted in cancers such as EC.METHODS:
Here, we evaluated the population of IL-10, TGF-ß, IFN-γ, and IL-17a-producing CD3+CD8+ T cells, their association with the circulating levels of miR-21 and miR-29b, and their diagnostic and/or prognostic (after 160 weeks of follow-up) utilities in 34 ESCC patients (12 newly diagnosed ND, 24 under-treatment UT) and 34 matched healthy donors.RESULTS:
The population of IL-10 and TGF-ß-producing CTLs (CD8+ Tregs) were considerably expanded, in addition to the overexpression of miR-21 in both groups (ND and UT) of ESCC patients, while the frequency of Tc17 and CD8+ Treg cells increased only in UT patients. The expression means of TGF-ß and IL-10 in CTLs were considered to be excellent biomarkers (1 ≥ area under the curve AUC ≥0.9) in distinguishing ESCC patients and associated subgroups from healthy subjects. Moreover, the lower expressions of TGF-ß, IL-17a, IL-10, and IFN-γ in CTLs were associated with ESCC better prognosis.CONCLUSIONS:
The association between the impaired function of CD3+ CD8+ T cell subsets and miR-21 expression could be introduced as novel therapeutic targets and powerful diagnostic and prognostic markers for ESCC.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Esophageal Neoplasms
/
T-Lymphocytes, Cytotoxic
/
MicroRNAs
/
Esophageal Squamous Cell Carcinoma
Type of study:
Diagnostic_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Humans
Language:
En
Journal:
J Clin Lab Anal
Journal subject:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Year:
2022
Document type:
Article
Affiliation country:
Iran