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Morphological and distributional properties of SMI-32 immunoreactive ganglion cells in the rat retina.
Tan, Huiying; Li, Xiaotao; Huang, Kang; Luo, Moxuan; Wang, Liping.
Affiliation
  • Tan H; Shenzhen Key Lab of Neuropsychiatric Modulation, Guangdong Provincial Key Laboratory of Brain Connectome and Behavior, CAS Center for Excellence in Brain Science and Intelligence Technology, Brain Cognition and Brain Disease Institute, Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundament
  • Li X; University of Chinese Academy of Sciences, Beijing, China.
  • Huang K; Shenzhen Key Lab of Neuropsychiatric Modulation, Guangdong Provincial Key Laboratory of Brain Connectome and Behavior, CAS Center for Excellence in Brain Science and Intelligence Technology, Brain Cognition and Brain Disease Institute, Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundament
  • Luo M; School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
  • Wang L; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
J Comp Neurol ; 530(8): 1276-1287, 2022 06.
Article in En | MEDLINE | ID: mdl-34802150
SMI-32 is widely used to identify entire populations of alpha retinal ganglion cells (RGCs), and several SMI-32+ RGC subsets have been studied thoroughly in rodents. However, due to the thick cover of SMI-32+ neurofilaments, the morphology of SMI-32+ RGCs in the central retinal region is obscured and rarely described. Moreover, SMI-32 labels more than one morphological RGC type and the full morphological characteristics and distribution of SMI-32+ RGCs have yet to be discovered. Here, using intracellular neurobiotin injections combined with SMI-32 antibody staining, we investigated morphological and distributional properties of the entire SMI-32+ RGCs population in the rat retina. We found that SMI-32+ RGCs were evenly distributed throughout the rat retina. We compared the morphological features of SMI-32+ ON and OFF cells in the central, middle, and peripheral retinal regions. We found that SMI-32+ RGCs in different regions have distinct characteristics, such as the soma area and the dendritic field area, and Sholl analysis of ON cells and OFF cells revealed significant differences between each region. We classified SMI-32+ RGCs into five clusters based on morphological features and found that a majority of SMI-32+ RGCs belong to alpha-like cells; however, a small proportion of SMI-32+ RGCs had small soma and small dendritic fields. Together, we present a full description of the morphology and distribution of SMI-32 immunoreactive RGCs in the rat retina.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Retina / Retinal Ganglion Cells Type of study: Prognostic_studies Limits: Animals Language: En Journal: J Comp Neurol Year: 2022 Document type: Article Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Retina / Retinal Ganglion Cells Type of study: Prognostic_studies Limits: Animals Language: En Journal: J Comp Neurol Year: 2022 Document type: Article Country of publication: United States