Protective Effects of the Hydroethanolic Extract of Fridericia chica on Undifferentiated Human Neuroblastoma Cells Exposed to α-Zearalenol (α-ZEL) and ß-Zearalenol (ß-ZEL).
Toxins (Basel)
; 13(11)2021 10 22.
Article
in En
| MEDLINE
| ID: mdl-34822532
ABSTRACT
Fridericia chica (Bignoniaceae) is a traditional medicinal plant. The aim of this research was to determine the protective effects of the hydroethanolic extract from the F. chica leaves (HEFc) against the cytotoxicity of zearalenone (α-ZEL) and ß-ZEL on SH-SY5Y cells. Free radical scavenging activity of HEFc was evaluated using the DPPH method. The cytotoxicity of both zearalenone metabolites and HEFc was examined using MTT test, as was the cytoprotective effects of the HEFc on cells treated with these mycotoxins. The chemical composition of HEFc was determined using UPLC-QTOF-MS/MS. HEFc elicited good DPPH radical scavenging activity following a concentration-dependent relationship. Cells exposed to α-ZEL exhibited a viability Ë50% after 48 h of treatment (25 and 50 µM), while those exposed to ß-ZEL showed viability Ë50% (100 µM) and Ë25% (25-100 µM) after 24 and 48 h of exposure, respectively. HEFc showed a significant increase in cell viability after exposure to α-ZEL (25 and 50 µM) and ß-ZEL (6-100 µM) (p < 0.05). UPLC-QTOF-MS/MS analyses allowed the identification of 10 phytochemical components in the HEFc. In short, the hydroethanolic extract of F. chica grown in Colombian Caribbean can protect against the effects of mycotoxins and it is a valuable source of compounds with antioxidant properties.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Zearalenone
/
Plant Extracts
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Protective Agents
/
Bignoniaceae
/
Neuroblastoma
Limits:
Humans
Language:
En
Journal:
Toxins (Basel)
Year:
2021
Document type:
Article
Affiliation country:
Colombia