Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis.
J Vet Med Sci
; 84(1): 129-132, 2022 Jan 24.
Article
in En
| MEDLINE
| ID: mdl-34853198
ABSTRACT
Taylorella equigenitalis causes contagious equine metritis. Here we compared seven nucleic acid amplification tests for T. equigenitalis to select a rapid and reliable diagnostic method. The 95% detection limits of each assay varied greatly real-time PCR had the lowest detection limit (0.77 fg/reaction); those of some of the conventional PCRs (cPCRs) were >100 fg/reaction. In experimentally infected samples, real-time PCR and semi-nested PCR showed the highest positive numbers (33 out of 42 samples), but two of the cPCRs detected only 2 and 7 positive results. Our results indicate that the use of sensitive molecular assays is important for the efficient detection of T. equigenitalis in clinical samples.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Gram-Negative Bacterial Infections
/
Taylorella equigenitalis
/
Horse Diseases
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
J Vet Med Sci
Journal subject:
MEDICINA VETERINARIA
Year:
2022
Document type:
Article
Affiliation country:
Japan