Lamin A/C-Dependent Translocation of Megakaryoblastic Leukemia-1 and ß-Catenin in Cyclic Strain-Induced Osteogenesis.
Cells
; 10(12)2021 12 14.
Article
in En
| MEDLINE
| ID: mdl-34944031
ABSTRACT
Lamins are intermediate filaments that play a crucial role in sensing mechanical strain in the nucleus of cells. ß-catenin and megakaryoblastic leukemia-1 (MKL1) are critical signaling molecules that need to be translocated to the nucleus for their transcription in response to mechanical strain that induces osteogenesis. However, the exact molecular mechanism behind the translocation of these molecules has not been fully investigated. This study used 10% cyclic strain to induce osteogenesis in the murine osteoblast precursor cell line (MC3T3). The translocation of ß-catenin and MKL1 was studied by performing knockdown and overexpression of lamin A/C (LMNA). Cyclic strain increased the expression of osteogenic markers such as alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), and enhanced ALP staining after seven days of incubation. Resultantly, MKL1 and ß-catenin were translocated in the nucleus from the cytoplasm during the stress-induced osteogenic process. Knockdown of LMNA decreased the accumulation of MKL1 and ß-catenin in the nucleus, whereas overexpression of LMNA increased the translocation of these molecules. In conclusion, our study indicates that both MKL1 and ß-catenin molecules are dependent on the expression of LMNA during strain-induced osteogenesis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteogenesis
/
Stress, Mechanical
/
Lamin Type A
/
Beta Catenin
Limits:
Animals
/
Humans
Language:
En
Journal:
Cells
Year:
2021
Document type:
Article
Affiliation country:
China