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Prevention of Postsurgical Abdominal Adhesion Using Electrospun TPU Nanofibers in Rat Model.
Gholami, Ahmad; Abdoluosefi, Homeira Emad; Riazimontazer, Elham; Azarpira, Negar; Behnam, Mohamadali; Emami, Farzin; Omidifar, Navid.
Affiliation
  • Gholami A; Biotechnology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
  • Abdoluosefi HE; Pharmaceutical Sciences Research Center, Shiraz University of Medical Science, Shiraz, Iran.
  • Riazimontazer E; Department of Nuclear Physics, Faculty of Physics, University of Kashan, Kashan, Iran.
  • Azarpira N; Biotechnology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
  • Behnam M; Department of Medicinal Chemistry, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
  • Emami F; Organ Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
  • Omidifar N; Nano Opto-Electronic Research Center, Electrical and Electronics Engineering Department, Shiraz University of Technology, Shiraz, Iran.
Biomed Res Int ; 2021: 9977142, 2021.
Article in En | MEDLINE | ID: mdl-34993249
Intra-abdominal adhesions following surgery are a challenging problem in surgical practice. This study fabricated different thermoplastic polyurethane (TPU) nanofibers with different average diameters using the electrospinning method. The conditions were evaluated by scanning electron microscopy (SEM), atomic force microscope (AFM), and Fourier transform infrared spectrometer (FTIR) analysis. A static tensile test was applied using a strength testing device to assess the mechanical properties of the electrospun scaffolds. By changing the effective electrospinning parameters, the best quality of nanofibers could be achieved with the lowest bead numbers. The electrospun nanofibers were evaluated in vivo using a rat cecal abrasion model. The macroscopic evaluation and the microscopic study, including the degree of adhesion and inflammation, were investigated after three and five weeks. The resultant electrospun TPU nanofibers had diameters ranging from about 200 to 1000 nm. The diameters and morphology of the nanofibers were significantly affected by the concentration of polymer. Uniform TPU nanofibers without beads could be prepared by electrospinning through reasonable control of the process concentration. These nanofibers' biodegradability and antibacterial properties were investigated by weight loss measurement and microdilution methods, respectively. The purpose of this study was to provide electrospun nanofibers having biodegradability and antibacterial properties that prevent any adhesions or inflammation after pelvic and abdominal surgeries. The in vivo experiments revealed that electrospun TPU nanofibers reduced the degree of abdominal adhesions. The histopathological study confirmed only a small extent of inflammatory cell infiltration in the 8% and 10% TPU. Conclusively, nanofibers containing 8% TPU significantly decreased the incidence and severity of postsurgical adhesions, and it is expected to be used in clinical applications in the future.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polyurethanes / Postoperative Complications / Tissue Adhesions / Abdominal Wall / Nanofibers Limits: Animals Language: En Journal: Biomed Res Int Year: 2021 Document type: Article Affiliation country: Iran Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polyurethanes / Postoperative Complications / Tissue Adhesions / Abdominal Wall / Nanofibers Limits: Animals Language: En Journal: Biomed Res Int Year: 2021 Document type: Article Affiliation country: Iran Country of publication: United States