Expression, purification and microscopic characterization of transmembrane BAX Inhibitor-1 motif containing protein 5.
Protein Expr Purif
; 193: 106045, 2022 05.
Article
in En
| MEDLINE
| ID: mdl-34999216
ABSTRACT
Transmembrane bax inhibitor-1 motif containing protein 5 (TMBIM5) is located on the inner membrane of mitochondria and is widely expressed in tissues but less frequently in the intestine and thymus. TMBIM5 affects mitochondrial cristae organization and is associated with Parkinson's disease. Here, we present the first report about expression, purification and the 2D classification projections derived from negatively stained electron micrographs of recombinant H. sapiens TMBIM5 (hTMBIM5). The described methods and results will support further structural and functional study of hTMBIM5.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mitochondrial Membranes
/
Mitochondria
Language:
En
Journal:
Protein Expr Purif
Journal subject:
BIOLOGIA MOLECULAR
Year:
2022
Document type:
Article
Affiliation country:
China