Surface sterilization for isolation of endophytes: Ensuring what (not) to grow.
J Basic Microbiol
; 62(6): 647-668, 2022 Jun.
Article
in En
| MEDLINE
| ID: mdl-35020220
ABSTRACT
Endophytic microbiota opens a magnificent arena of metabolites that served as a potential source of medicines for treating a variety of ailments and having prospective uses in agriculture, food, cosmetics, and many more. There are umpteen reports of endophytes improving the growth and tolerance of plants. In addition, endophytes from lifesaving drug-producing plants such as Taxus, Nothapodytes, Catharanthus, and so forth have the ability to produce host mimicking compounds. To harness these benefits, it is imperative to isolate the true endophytes, not the surface microflora. The foremost step in endophyte isolation is the removal of epiphytic microbes from plant tissues, called as surface sterilization. The success of surface sterilization decides "what to grow" (the endophytes) and "what not to grow" (the epiphytes). It is very crucial to use an appropriate sterilant solution, concentration, and exposure time to ensure thorough surface disinfection with minimal damage to the endophytic diversity. Commonly used surface sterilants include sodium hypochlorite (2%-10%), ethanol (70%-90%), mercuric chloride (0.1%), formaldehyde (40%), and so forth. In addition, the efficiency could further be improved by pretreatment with surfactants such as Triton X-100, Tween 80, and Tween 20. This review comprehensively deals with the various sterilants and sterilization methods for the isolation of endophytic microbes. In addition, the mechanisms and rationale behind using specific surface sterilants have also been elaborated at length.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plants, Medicinal
/
Taxus
Type of study:
Observational_studies
Language:
En
Journal:
J Basic Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2022
Document type:
Article
Affiliation country:
India