Identification of key biomarkers in steroid-induced osteonecrosis of the femoral head and their correlation with immune infiltration by bioinformatics analysis.
BMC Musculoskelet Disord
; 23(1): 67, 2022 Jan 18.
Article
in En
| MEDLINE
| ID: mdl-35042504
ABSTRACT
OBJECTIVE:
This study aimed to identify key diagnostic markers and immune infiltration of (SONFH) by bioinformatics analysis.METHODS:
Related SONFH datasets were downloaded from the Gene Expression Omnibus (GEO) database. First, we identified the differentially expressed genes (DEGs) and performed the functional enrichment analysis. Then weighted correlation network analysis (WGCNA) and the MCODE plug-in in Cytoscape were used to identify the diagnostic markers of SONFH. Finally, CIBERSORT was used to analyze the immune infiltration between SONFH and healthy controls, and the correlation between infiltrating immune cells and diagnostic markers was analyzed.RESULTS:
TYROBP, TLR2, P2RY13, TLR8, HCK, MNDA, and NCF2 may be key diagnostic markers of SONFH. Immune cell infiltration analysis revealed that Memory B cells and activated dendritic cells may be related to the SONFH process. Moreover, HCK was negatively correlated with CD8 T cells, and neutrophils were positively correlated with those key diagnostic markers.CONCLUSIONS:
TYROBP, TLR2, P2RY13, TLR8, HCK, MNDA, and NCF2 may be used as diagnostic markers of SONFH, and immune-related mechanism of SONFH and the potential immunotherapy are worthy of further study.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteonecrosis
/
Femur Head
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
BMC Musculoskelet Disord
Journal subject:
FISIOLOGIA
/
ORTOPEDIA
Year:
2022
Document type:
Article
Affiliation country:
China