Integrating Biocatalysis with Viscous Deep Eutectic Solvents in Lab-On-A-Chip Microreactors.
ChemSusChem
; 15(9): e202102674, 2022 May 06.
Article
in En
| MEDLINE
| ID: mdl-35084121
ABSTRACT
The combination of deep eutectic solvents (DESs, ChCl/glycerol 1 2) with buffer (up to 15 % v/v) leads to solvent mixtures that exert viscosities below 25â
mPa s-1 at 45 °C while keeping their non-aqueous nature. This enables the setup of efficient enzymatic esterifications, which can also be applied in different continuous systems. Following those premises, the use of microreactors in biocatalytic reactions was explored using (low-viscous) DES-buffer media, showing that reactions could be performed efficiently. Under non-optimized conditions, the microreactor devices led to specific productivities considerably higher than those observed in the batch reactor (14 vs. 0.24â
mgproduct min-1 mgbiocat -1 ) at the same enzyme loadings and conversion of 6 % (to assure a fair comparison). Looking beyond, the combination of several microchannels (e. g., in scale-out fashion) with DES-water media may lead to powerful, sustainable, and efficient tools for industrial synthesis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Deep Eutectic Solvents
/
Lipase
Language:
En
Journal:
ChemSusChem
Journal subject:
QUIMICA
/
TOXICOLOGIA
Year:
2022
Document type:
Article
Affiliation country:
Chile