Recellularized rat testis scaffolds with embryoid bodies cells: a promising approach for tissue engineering.
Syst Biol Reprod Med
; 68(1): 44-54, 2022 Feb.
Article
in En
| MEDLINE
| ID: mdl-35086406
ABSTRACT
Tissue engineering is gaining use to investigate the application of its techniques for infertility treatment. The use of pluripotent embryonic cells for in vitro production of viable spermatozoa in testicular scaffolds is a promising strategy that could solve male infertility. Due to cell-extracellular matrix (ECM) interactions, here we aim to investigate the differentiation of embryoid bodies (EBs) in cultured into decellularized rat testis scaffolds. Decellularized testis (P = 0.019) with a low concentration of gDNA (30.58 mg/ng tissue) was obtained by sodium dodecyl sulfate perfusion. The structural proteins (collagens type I and III) and the adhesive glycoproteins of ECM (laminin and fibronectin) were preserved according to histological and scanning electron microscopy (SEM) analyses. Then, decellularized rat testis were cultured for 7 days with EB, and EB mixed with retinoic acid (RA) in non-adherent plates. By SEM, we observe that embryonic stem cells adhered in the decellularized testis ECM. By immunofluorescence, we verified the positive expression of HSD17B3, GDNF, ACRV-1, and TRIM-36, indicating their differentiation using RA in vitro, reinforcing the possibility of EB in male germ cell differentiation. Finally, recellularized testis ECM may be a promising tool for future new approaches for testicular cell differentiation applied to assisted reproduction techniques and infertility treatment.Abbreviations ACRV-1 Acrosomal vesicle protein 1; ATB Penicillin-streptomycin; DAPI 4,6-Diamidino-2-phenylindole; EB Embryoid bodies; ECM Extracellular matrix; ESCs Pluripotent embryonic stem cells; GAGs Glycosaminoglycans; gDNA Genomic DNA; GDNF Glial cell line-derived neurotrophic factor; H&E Hematoxylin and eosin; HSD17B3 17-beta-Hydroxysteroid dehydrogenase type 3; PBS Phosphate-buffered saline; PGCLCs Primordial germ-cell-like cells; RA Retinoic acid; SDS Sodium dodecyl sulfate; SEM Scanning electron microscopy; SSCs Spermatogonial stem cells; TRIM-36 Tripartite Motif Containing 36.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tissue Engineering
/
Embryoid Bodies
Limits:
Animals
Language:
En
Journal:
Syst Biol Reprod Med
Journal subject:
MEDICINA REPRODUTIVA
/
UROLOGIA
Year:
2022
Document type:
Article
Affiliation country:
Brazil
Publication country:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM