Regulatory effect of Panax notoginseng saponins on the oxidative stress and histone acetylation induced by porcine circovirus type 2.
J Vet Med Sci
; 84(4): 600-609, 2022 Apr 15.
Article
in En
| MEDLINE
| ID: mdl-35125373
ABSTRACT
Porcine circovirus type 2 (PCV2) exists widely in swine populations worldwide, and healthy PCV2 virus carriers have enhanced the severity of the infection, which is becoming more difficult to control. This study investigated the regulatory effect of Panax notoginseng saponins (PNS) on the oxidative stress and histone acetylation modification induced by PCV2 in vitro and in mice. In vitro, PNS significantly increased the scavenging capacities of superoxide anion radicals (O2â¢-) and hydroxyl radicals (â¢OH) and reduced the content of hydrogen peroxide (H2O2) induced by PCV2 in porcine alveolar macrophages (3D4/2). In addition, PNS decreased the protein expression level of histone H4 acetylation (Ac-H4) by increasing the activity of histone deacetylase (HDAC) in PCV2-infected 3D4/2 cells. In vivo, PNS enhanced the scavenging capacities of â¢OH and O2â¢- and reduced the content of H2O2 in the spleens of PCV2-infected mice. PNS also reduced the protein expression level of histone H3 acetylation (Ac-H3) by reducing the activity of histone acetylase (HAT) and increasing the activity of HDAC in the spleens of PCV2-infected mice. PCV2 infection activated oxidative stress and histone acetylation in vitro and in mice, but PNS ameliorated this oxidative stress. The research can provide experimental basis for exploring the antioxidant effect and the regulation of histone acetylation of PNS on PCV2-infected 3D4/2 cells and mice in vitro and in vivo, and provide new ideas for the treatment of PCV2 infection.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rodent Diseases
/
Saponins
/
Swine Diseases
/
Circovirus
/
Circoviridae Infections
/
Panax notoginseng
Limits:
Animals
Language:
En
Journal:
J Vet Med Sci
Journal subject:
MEDICINA VETERINARIA
Year:
2022
Document type:
Article
Affiliation country:
China