The active ingredient (DSH-20) of Salvia miltiorrhiza flower reduces oxidative damage and apoptosis in cardiomyocytes by regulating miR-1.
Mol Biol Rep
; 49(5): 3675-3684, 2022 May.
Article
in En
| MEDLINE
| ID: mdl-35179668
ABSTRACT
BACKGROUND:
DSH-20, the active ingredient of Salvia miltiorrhiza flower extract, is used to treat cardiovascular diseases. However, its mechanism of action remains unclear. Herein, we investigated the intervention of DSH-20 in H2O2-induced oxidative damage and apoptosis in cardiomyocytes. METHODS ANDRESULTS:
H2O2 was used to induce oxidative damage and apoptosis in H9c2 cardiomyocytes. Based on concentration gradient studies, we found that 62.5 µg/mL DSH-20 significantly reduced reactive oxygen species and lactate dehydrogenase levels and increased superoxide dismutase levels. DSH-20 also alleviated the apoptosis rate, the changes in mRNA of apoptosis-related genes (Bcl-2, BAX, and Caspase-3) and miR-1 expression. Moreover, transfection of miR-1 mimics aggravated oxidative damage and apoptosis, whereas DSH-20 alleviated these effects.CONCLUSIONS:
DSH-20 reduced H2O2-induced oxidative damage and apoptosis in H9c2 cardiomyocytes likely by downregulating miR-1 expression.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Salvia miltiorrhiza
/
MicroRNAs
Language:
En
Journal:
Mol Biol Rep
Year:
2022
Document type:
Article
Affiliation country:
China