Your browser doesn't support javascript.
loading
Characterizing human mesenchymal stromal cells' immune-modulatory potency using targeted lipidomic profiling of sphingolipids.
DeVeaux, S'Dravious A; Ogle, Molly E; Vyshnya, Sofiya; Chiappa, Nathan F; Leitmann, Bobby; Rudy, Ryan; Day, Abigail; Mortensen, Luke J; Kurtzberg, Joanne; Roy, Krishnendu; Botchwey, Edward A.
Affiliation
  • DeVeaux SA; The Wallace H. Coulter Department of Biomedical Engineering, Georgia Tech and Emory, Atlanta, GA; Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA.
  • Ogle ME; The Wallace H. Coulter Department of Biomedical Engineering, Georgia Tech and Emory, Atlanta, GA; Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA.
  • Vyshnya S; The Wallace H. Coulter Department of Biomedical Engineering, Georgia Tech and Emory, Atlanta, GA; Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA.
  • Chiappa NF; The Wallace H. Coulter Department of Biomedical Engineering, Georgia Tech and Emory, Atlanta, GA; Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA.
  • Leitmann B; Regenerative Bioscience Center, Rhodes Center for ADS, University of Georgia, Athens, GA; School of Chemical, Materials and Biomedical Engineering, University of Georgia, Athens, GA.
  • Rudy R; The Wallace H. Coulter Department of Biomedical Engineering, Georgia Tech and Emory, Atlanta, GA; Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA.
  • Day A; The Wallace H. Coulter Department of Biomedical Engineering, Georgia Tech and Emory, Atlanta, GA; Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA.
  • Mortensen LJ; Regenerative Bioscience Center, Rhodes Center for ADS, University of Georgia, Athens, GA; School of Chemical, Materials and Biomedical Engineering, University of Georgia, Athens, GA.
  • Kurtzberg J; Marcus Center for Cellular Cures, Duke University School of Medicine, Durham, NC.
  • Roy K; The Wallace H. Coulter Department of Biomedical Engineering, Georgia Tech and Emory, Atlanta, GA; Marcus Center for Therapeutic Cell Characterization and Manufacturing, Georgia Institute of Technology, Atlanta, GA; NSF Engineering Research Center (ERC) for Cell Manufacturing Technologies (CMaT), Geo
  • Botchwey EA; The Wallace H. Coulter Department of Biomedical Engineering, Georgia Tech and Emory, Atlanta, GA; Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA. Electronic address: edward.botchwey@bme.gatech.edu.
Cytotherapy ; 24(6): 608-618, 2022 06.
Article in En | MEDLINE | ID: mdl-35190267
Cell therapies are expected to increase over the next decade owing to increasing demand for clinical applications. Mesenchymal stromal cells (MSCs) have been explored to treat a number of diseases, with some successes in early clinical trials. Despite early successes, poor MSC characterization results in lessened therapeutic capacity once in vivo. Here, we characterized MSCs derived from bone marrow (BM), adipose tissue and umbilical cord tissue for sphingolipids (SLs), a class of bioactive lipids, using liquid chromatography/tandem mass spectrometry. We found that ceramide levels differed based on the donor's sex in BM-MSCs. We detected fatty acyl chain variants in MSCs from all three sources. Linear discriminant analysis revealed that MSCs separated based on tissue source. Principal component analysis showed that interferon-γ-primed and unstimulated MSCs separated according to their SL signature. Lastly, we detected higher ceramide levels in low indoleamine 2,3-dioxygenase MSCs, indicating that sphingomyelinase or ceramidase enzymatic activity may be involved in their immune potency.
Subject(s)
Key words
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sphingolipids / Mesenchymal Stem Cells Limits: Humans Language: En Journal: Cytotherapy Journal subject: TERAPEUTICA Year: 2022 Document type: Article Country of publication: United kingdom
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sphingolipids / Mesenchymal Stem Cells Limits: Humans Language: En Journal: Cytotherapy Journal subject: TERAPEUTICA Year: 2022 Document type: Article Country of publication: United kingdom