A Primer for Single-Cell Sequencing in Non-Model Organisms.
Genes (Basel)
; 13(2)2022 02 19.
Article
in En
| MEDLINE
| ID: mdl-35205423
ABSTRACT
Single-cell sequencing technologies have led to a revolution in our knowledge of the diversity of cell types, connections between biological levels of organization, and relationships between genotype and phenotype. These advances have mainly come from using model organisms; however, using single-cell sequencing in non-model organisms could enable investigations of questions inaccessible with typical model organisms. This primer describes a general workflow for single-cell sequencing studies and considerations for using non-model organisms (limited to multicellular animals). Importantly, single-cell sequencing, when further applied in non-model organisms, will allow for a deeper understanding of the mechanisms between genotype and phenotype and the basis for biological variation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phenotype
Limits:
Animals
Language:
En
Journal:
Genes (Basel)
Year:
2022
Document type:
Article
Affiliation country:
United States