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Expansion Microscopy of Larval Zebrafish Brains and Zebrafish Embryos.
Perelsman, Ory; Asano, Shoh; Freifeld, Limor.
Affiliation
  • Perelsman O; Department of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel.
  • Asano S; Internal Medicine Research Unit, Pfizer, Cambridge, MA, USA.
  • Freifeld L; Department of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel. freifeld@bm.technion.ac.il.
Methods Mol Biol ; 2440: 211-222, 2022.
Article in En | MEDLINE | ID: mdl-35218542
Since its introduction in 2015, expansion microscopy (ExM) allowed imaging a broad variety of biological structures in many models, at nanoscale resolution. Here, we describe in detail a protocol for application of ExM in whole-brains of zebrafish larvae and intact embryos, and discuss the considerations involved in the imaging of nonflat, whole-organ or organism samples, more broadly.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Zebrafish / Microscopy Limits: Animals Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2022 Document type: Article Affiliation country: Israel Country of publication: United States
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Zebrafish / Microscopy Limits: Animals Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2022 Document type: Article Affiliation country: Israel Country of publication: United States