MiR-532-3p suppresses cell proliferation, migration and invasion of colon adenocarcinoma via targeting FJX1.

OBJECTIVE: We attempted to probe into mechanism of FJX1 regulating cell behaviors of colon adenocarcinoma, and to provide ideas for targeted therapy of colon adenocarcinoma. METHODS: Differential mRNAs were screened out from TCGA-COAD dataset. K-M analysis was done to compare correlation between FJX1 expression and patient's survival status. Upstream miRNAs of FJX1 were identified by bioinformatics methods. Targeted relationship of miR-532-3p and FJX1 was verified by dual-luciferase reporter gene assay. FJX1 level in colon adenocarcinoma cell lines was assayed via qRT-PCR and western blot. The impact of regulation of miR-532-3p to FJX1 on biological functions of colon adenocarcinoma cells was evaluated by MTT, wound healing and Transwell invasion assays. RESULTS: TCGA-COAD data and qRT-PCR manifested that FJX1 was increased in colon adenocarcinoma tissue, while miR-532-3p was conspicuously less expressed. Survival analysis denoted that FJX1 is potential to be an independent prognosticator in colon adenocarcinoma. Dual-luciferase assay manifested that miR-532-3p targeted FJX1 to repress expression of FJX1. Overexpression of FJX1 could stimulate cell malignant behaviors of colon adenocarcinoma, whereas forced expression of miR-532-3p reversed this promotive effect. CONCLUSION: This study revealed that FJX1 was an oncogene in colon adenocarcinoma cells, which was regulated by miR-532-3p. MiR-532-3p targeted and regulated FJX1 expression to suppress cell malignant behaviors of colon adenocarcinoma.