Mechanistic insights into the amelioration effects of lipopolysaccharide-induced acute lung injury by baicalein: An integrated systems pharmacology study and experimental validation.
Pulm Pharmacol Ther
; 73-74: 102121, 2022 06.
Article
in En
| MEDLINE
| ID: mdl-35283292
ABSTRACT
BACKGROUND:
Acute lung injury is an acute progressive respiratory failure caused by several of non-cardiogenic factors which involves in excessive amplification or uncontrolled inflammatory response.OBJECTIVES:
In this study, we investigated the protective effect of baicalein against acute lung injury induced by LPS and explored the underlying mechanisms.METHODS:
Forty-eight SPF male C57BL/6 mice were randomly divided into normal group, model group, dexamethasone group and baicalein low-dose, medium-dose and high-dose groups. After 5 days of adaptive feeding, the mice were intraperitoneally injected with LPS and dissected after 12 h. Hematoxylin-eosin staining, ELISA assay, immunofluorescence assay and Western-Blot were applied to appraise microstructural changes and protein expressions of lung tissues. Systems pharmacology study was used to evaluate the protection of baicalein on acute lung injury.FINDINGS:
The results showed that baicalein administration could significantly inhibit LPS-induced lung morphological changes, inhibit inflammatory response and pyroptosis. A total of forty-three potential targets of baicalein and acute lung injury were obtained. And PI3K-Akt, TNF and NF-κB were mainly signaling pathways. It is worth mentioning that this experiment also confirmed that NLRP3, caspase-1 and other inflammasome are involved in pyroptosis.CONCLUSION:
Baicalein has protected against LPS-induced lung tissues injury via inhibiting inflammatory response and pyroptosis.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Lipopolysaccharides
/
Acute Lung Injury
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Pulm Pharmacol Ther
Journal subject:
FARMACOLOGIA
Year:
2022
Document type:
Article