Imaging and characterization of transitions in biofilm morphology via anomalous diffusion following environmental perturbation.
Biomed Opt Express
; 13(3): 1654-1670, 2022 Mar 01.
Article
in En
| MEDLINE
| ID: mdl-35414993
ABSTRACT
Microorganisms form macroscopic structures for the purpose of environmental adaptation. Sudden environmental perturbations induce dynamics that cause bacterial biofilm morphology to transit to another equilibrium state, thought to be related to anomalous diffusion processes. Here, detecting the super-diffusion characteristics would offer a long-sought goal for a rapid detection method of biofilm phenotypes based on their dynamics, such as growth or dispersal. In this paper, phase-sensitive Doppler optical coherence tomography (OCT) and dynamic light scattering (DLS) are combined to demonstrate wide field-of-view and label-free internal dynamic imaging of biofilms. The probability density functions (PDFs) of phase displacement of the backscattered light and the dynamic characteristics of the PDFs are estimated by a simplified mixed Cauchy and Gaussian model. This model can quantify the super-diffusion state and estimate the dynamic characteristics and macroscopic responses in biofilms that may further describe dispersion and growth in biofilm models.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Type of study:
Prognostic_studies
Language:
En
Journal:
Biomed Opt Express
Year:
2022
Document type:
Article
Affiliation country:
United States